High Throughput Platform for CRISPR-Cas9 Editing with Judd Hultquist, Northwestern University

High Throughput Platform for CRISPR-Cas9 Editing

Judd Hultquist, Assistant Professor, Feinberg School of Medicine at Northwestern University describes his approach at Phacilitate Leaders World 2020 in Miami, FL.
Of all the data, insights and findings in this 16-minute presentation, there is one stark conclusion…
 
High throughput gene editing in primary human cells is doable.
You should be doing it.

But how?

We begin with a mission statement 'to improve patient health, we need to invest in curative and improved therapeutic interventions' and these interventions need to account for the host as much as the pathogen.

Judd’s lab teamed up with leaders at UCSF and UC Berkeley to develop a high throughput CRISPR-Cas9 platform for editing in human T cells, whereby the end results are modified T cells that can be used for downstream phenotypic assays for HIV infection. A wealth of data is presented on different knock out scenarios and then Judd walks you through his team’s strategy for high throughput, arrayed CRISPR knock out in primary CD4+ T cells.

Watch the video to find out more.